She then started to produce low-performances until March 2016, when she won her second singles title at Sharm El Sheikh, and two weeks later another event at the same venue.

She then made her WTA Tour main-draw debut at the 2017 Korea Open after defeating two low-ranked players, and then lost in the first round of the main draw to Priscilla Hon.

[12] She then at the Premier-level Qatar Ladies Open made her first WTA Tour quarterfinal by defeating Samantha Stosur and Hsieh Su-wei, but then lost to seed No.

[7][13] At the Miami Open, she debuted at the Premier Mandatory level-tournaments but was eliminated in the second round by Angelique Kerber.

[16] In June, she beat Anett Kontaveit to reach the second round of the French Open,[17] where she lost to Irina-Camelia Begu.

[18] In July, she reached quarterfinals of the Wimbledon Championships, beating third seed Karolína Plíšková 13–11 in the final set of their fourth-round match, which lasted over three hours.

[23] Muchová then continued with good results, reaching semifinals of the Premier-level Kremlin Cup, in which she lost to Anastasia Pavlyuchenkova.

[24] At the end of the year, she debuted at the WTA Elite Trophy with success in her round-robin group, defeating two Americans, Sofia Kenin and Alison Riske,[25] but later lost to Aryna Sabalenka in the semifinal.

She reached the second round of the Australian Open for the first time in her career but lost to CiCi Bellis in straight sets.

[27] She then played for the first time at the Qatar Ladies Open, where she also recorded her first win over Magda Linette,[7] but lost to seventh seed Kiki Bertens in round two.

[28] After the six month suspension of the WTA Tour due to COVID-19 pandemic, she first played at the Cincinnati Open where she beat qualifier Ann Li in the first round,[7] before she lost to Naomi Osaka.

[29] Her best performance of the season came at the US Open, when she beat Venus Williams[30] Anna Kalinskaya, and Sorana Cîrstea[31][32] to reach her first round of 16 there before she lost to Victoria Azarenka.

[34][35] The 22nd seeded Muchová was ousted in the first round of the US Open by Sara Sorribes Tormo, in straight sets.

In Madrid, using protected ranking, she defeated Chinese teenager Zheng Qinwen, before losing to 11th seed Belinda Bencic.

[54] In Cincinnati, she reached the final of a WTA 1000 for the first time, losing to sixth seed Coco Gauff in two sets but she recorded a career-high ranking of No.

[55] At the US Open, Muchová again hit a good form by reaching the semifinals of the tournament for the first time in her career and second at a major in the season.

[62] Muchová qualified for the end-of-season WTA Finals for the first time, but was forced to withdraw from the tournament less than a week before it began due to a right wrist injury she suffered at the US Open.

[63] Muchová withdrew from the Australian Open[64] and in February, underwent surgery which made her skip the Middle Eastern swing and the USA Sunshine Double tournaments extending her hiatus.

[65] She made her return to the WTA Tour in June at the Eastbourne International, where she recorded her first win against Magda Linette.

[68] In Palermo, Muchová entered as a wildcard and defeated Irina-Camelia Begu in the last four to reach her first final since August 2023.

[84] Muchová continued her good form at the Ningbo Open, overcoming qualifier Olivia Gadecki,[85] Jacqeline Cristian[86] and seventh seed Anna Kalinskaya to reach the semifinals,[87] where she retired due to injury, after losing the first set to Mirra Andreeva.

Throughout 2019, her breakout season, she was praised for her style of play by Mats Wilander, who stated that she could "achieve great things".

With her on-court demeanour, fluid style, grace, and variety, she has been compared by some commentators to Justine Henin and Roger Federer.

[citation needed] Muchová has had several coaches in her career: Emil Miške (2017–2019, Apr 2023–), David Kotyza (2020–Aug 2022), Jan Blecha (Sep 2022–Apr 2023), and Kirsten Flipkens (2023–).