[12] The Ninja 300 also has 5 spoke wheels, neutral finder, and optional antilock braking system (ABS).

[13][14] By comparison with the 250R, the Ninja 300 also has a slightly smaller fuel tank, taller gearing,[15] and a back-torque-limiting slipper clutch with an assist mechanism that decreases clutch lever effort.

[4] Despite being a sport bike, it has comfortable ergonomics (such as windscreen effectively protecting from wind blast) which promotes commuting.

[16] Dynamometer tests showed that the Ninja 300 produces more power with 39.95 hp (29.79 kW) compared with the 250's 25.48 hp (19.00 kW), and higher torque across the rev range at 18 to 13.57 lb⋅ft (24.40 to 18.40 N⋅m).

[1][2] The Ninja 300 competes with (and is commonly compared with) other entry-level (300/250 cc) bikes such as Yamaha R3, KTM RC390, Suzuki GSX250R and Honda CBR300R.