She is known for her role as Lindsay on You're the Worst,[1] as a cappella leader Alice in Pitch Perfect, and as the exocomp Peanut Hamper on Star Trek: Lower Decks.

[2][3] Donohue grew up in the Lower East Side neighborhood of Manhattan, New York City,[2] and went to a performing arts high school.

[5] In 2008, she graduated from Fordham University where she was a communication and media studies major with a concentration in film.

Donohue was also featured in the video for "Coder Girl" by Dale Chase.

In January 2016, Donohue appeared in the role of Jan in the Fox presentation of Grease Live.