After the opening of the Kharkov Imperial University (now Karazin University), the collegium was closed, and the teaching staff was transferred to the newly established seminary with a six-year term of study for persons of the clergy.

In 1726, Bishop Epiphanius of Belgorod, at the request of Prince Mikhail Golitsyn, transferred the Slavic-Greek-Latin theological school, founded in 1721 and located at the St. Nikolaus Belgorod Monastery [ru], to the town of Kharkov.

In 1734, the school, which was supposed to teach representatives of all classes, received the status of Collegium.

[1] The stone building in this area was built in 1851 according to the design of the St. Petersburg German-born architect Andrey Ton [ru].

In Soviet times, a school of red officers was opened in the seminary building.