In 2013, Gamblin began appearing in Neighbours as Brad Willis, making him the third actor to play the role after Benjamin Grant Mitchell and Scott Michaelson.

In 2000 he was chosen to perform as the sole male dancer presenting Barbra Streisand on stage for her Australian tour of Timeless.

[1] From January 2003 to November 2005 Gamblin played the role of Scott Hunter in the Australian television soap opera Home and Away for which he won the Logie Award for Most Popular New Male Talent in 2004.

[1] He also made an appearance as Greg Fallon in Holby City, when the character was one of the paramedics who attended the scene of Mark and Tricia Williams' car accident.

[1] In 2012, Gamblin portrayed the role of Rick Taylor in the Australian television drama series Tricky Business which lasted for one season.