Kitatsuru (北都留郡, Kitatsuru-gun) is a rural district located in northeastern Yamanashi Prefecture, Japan.

As of October 2020, the district had an estimated population of 1,214 with a density of 7.879 persons per km2.

[1] Prior to its dissolution, the district consisted of only two villages: The district formerly included the city of Ōtsuki and a portion of the city of Uenohara.

Kitatsuru District was founded during the early Meiji period establishment of the municipalities system on July 22, 1878 and initially consisted of 18 villages.

Uenohara was elevated to town status on December 27, 1897, followed by Ōtsuki on April 1, 1933.