In 1570s a renowned businessman and benefactor of the ghetto, Mordechai Maisel, decided to build in the area of the present Klausen Synagogue a kloyz, or complex of buildings, probably including synagogues and a private Talmudic school.

In 1694, the building was finished and two years later monumental three-tiered aron ha-kodesh, the Torah Ark, was added, thanks to the endowment of Samuel Oppenheimer, an affluent and influential personality of the Austrian monarchy, part of which Prague was at the time.

[2] On 11 October, 2024, the synagogue held a Kol Nidre service, led by Rabbi David Maxa of the Czech community of Progressive Jews, for the first time since World War II.

[3] About a decade later, during years 1995–96, the synagogue was restored again and the topic of Jewish festivals and customs returned to the exposition.

Visitors are invited to get acquainted with the foundational texts of Judaism (Torah and Talmud), sacral space of Judaism (traditional components of the synagogue interior, order of synagogue prayer service and texts and objects used during it, etc.).