Knowsley is a locality in the City of Greater Bendigo, Victoria.

It is located 134 kilometres (83 mi) north of the state capital, Melbourne.

[1] Knowsley began to develop in 1876 with the opening of the Moorabbee Hotel, followed by a school in 1879.

Its early economy centered on timber cutting, particularly firewood, which was transported to Bendigo.

By 1933, Knowsley was a small farming and grazing township with a school, church, recreation hall, and hotel, supporting a population of around 150.