The Knoxville Challenger is a professional tennis tournament played on indoor hard courts.

It is held annually in Knoxville, Tennessee, United States, since 2000.

The Knoxville Challenger is held at Goodfriend Tennis Center on the campus of the University of Tennessee.

The tournament is the second of three indoor Challenger tournaments at college venues to end the USTA Pro Circuit season.

The Charlottesville Men's Pro Challenger (University of Virginia) is held the week before, and the Champaign Challenger (University of Illinois) is the week after.