The Kyoto Botanical Garden (京都府立植物園, Kyōto Furitsu Shokubutsuen, 240,000 m²), also known as the Kyoto Prefectural Botanical Garden, is a major botanical garden with conservatory located next to the Kamo River, Hangi-cho Simogamo, Sakyō-ku, Kyoto, Japan.

When World War II ended, the garden was designated a garrison by Occupation forces and was seized in 1946.

The garden also contains a very substantial conservatory complex (4,694 m2) containing about 25,000 specimens representing 4,500 species.

It is a set of rooms shaped to resemble the nearby Kinkaku-ji Temple and Kyoto's northern mountains, built of glass with iron frames, and opened in 1992.

The garden is located in northern part of Kyoto city and accessible by public transports.