In the charter there is a mention of three Roman Catholic churches existing in the Lašva Valley,[citation needed] although only one can be confirmed by the gifting of the Bosnian Ban Matthew Ninoslaus in 1250.

With the arrival of the new oppressive and bloodthirsty masters from the East, people began to flee over the borders to the south and north, while others sought refuge in mountains led by the Franciscans.

"[page needed] The last battle against Ottoman expansion in the Lašva Valley was lost on May 1463, when Mehmed the Conqueror on his return from Jajce defeated the last Bosnian king Stephen Tomašević at Milodraž in today's Brestovsko; located between Kiseljak and Busovača.

[5] Upon the Ottoman conquest, the plaintiff of the Franciscans Anđeo Zvizdović advised the Sultan to stop forcing the people of Bosnia to flee because of religious persecution.

As a result, Travnik began to grow and emerge as an integral part of the Ottoman Empire's jurisdiction over the Lašva Valley and Bosnia.

The modernization of the Austro-Hungarian rule transformed the Lašva Valley into a capable area worthy of competing against larger and more established regions.

[citation needed] The region saw fierce fighting between the Croatian Defence Council and the Army of the Republic of Bosnia and Herzegovina during the Croat-Bosniak War.