"Ladies and Gentlemen" peaked at numbers 2 and 25 on both the Billboard Mainstream Rock and Alternative Songs charts respectively, and is also the band's best-selling single, being certified Gold by the Recording Industry Association of America (RIAA) in 2010.

[2] PopMatters contributor Andrew Blackie found it "painfully unnecessary", describing it as "[A] third-rate downtuned distortion-fest, the verses aren’t so much sung as spoken... in a monotonous swagger.

"[3] Conversely, Clark Collis of Entertainment Weekly praised Saliva for infusing the song with a "bright, leavening pop sensibility.

"[4] Directed by Stephen Penta, the video takes place in a ring where two former Suicide Girls models are boxing each other, while lead singer Josey Scott is playing "multiple roles including announcer, trainer and referee.

It was also used the theme for the 2007 annual AST Dew Tour, Universe of Entertainment commercials for the Sony PlayStation 3 and the television spots for the 2009 film Star Trek.

The song is used as the background music to the intro video for the Ohio State Buckeyes men's ice hockey 2010 season during home games.