The Lady's Well is a natural spring surmounted by a large cross that stands beside the Stra Burn Ford (NS254630) near Auchmannoch House in the Parish of Sorn, East Ayrshire, Scotland.

[7] The Ordnance Survey may contradict the fine detail of the story that Mary Queen of Scots watered her horses here once by stating that the 'Queen's Syke "..is a Small Stream or marsh forming a Junction with the Stra Burn about 13 chains N [North] east of Coplar.

[11] A substantial stone trough has been set into the ground with the cross at the top and a narrow secondary overflow appears to be present in addition to the aforementioned pipe that leads to the Stra Burn.

The construction of a new road nearby to Crofthead Farm, avoiding the passage through the Stra Burn Ford, cut off the water supply to this natural spring.

Other offerings were also sometimes made at such sites, including coins, pins, white pebbles, etc[15] as is still the case at Saint Queran's Holy Well, Islesteps, near Dumfries.