Lake Tota (Spanish: Lago de Tota) is the largest lake in Colombia, located in the east of Boyacá department, inside the Sugamuxi Province[1], it is the source of the Upia River which flows into the Orinoco River basin.

The name Tota comes from the Chibcha language of the Muisca and according to linguist Mariana Escribano its main name refers to "Astronomic Observatory", relating to its sacred uses for that culture.

The average water temperature of 13 °C allowed for the introduction of rainbow trout (Onchorynchus mykiss) which created a valuable wild fishery (a mounted specimen in Restaurante Lucho, Aquitania, reportedly early in the 1990s, weighed 7 kg) and cage culture aquaculture, but predation on native fish species as led to the probable extinction of the pez graso or grease fish (Rhizosomichthys totae) believed native to only this lake.

Lake Tota is a breeding ground for several threatened or endangered bird species, including the least bittern (Ixobrychus exilis bogotensis), the Colombian ruddy duck (Oxyura jamaicensis andina), the Bogotá rail (Rallus semiplumbeus) and Apolinar's wren (Cistothorus apolinari).

The last confirmed sighting of the Colombian grebe (Podiceps andinus), now considered to be extinct, was at Lake Tota.