The Landover Subdivision is a railroad line owned and operated by CSX Transportation.

At the Landover end, the line connects to the Amtrak Northeast Corridor (NEC).

CSX operates freight trains on an 8.3 miles (13.4 km) section of the NEC between Landover and its Popes Creek Subdivision.

[3]: 335  At that time the branch included the Anacostia Railroad Bridge and the Virginia Avenue Tunnel, and the route continued past the tunnel to the B&P passenger station at Sixth Street and B Street NW and the Long Bridge.

Subsequently, the PRR used the old route only for freight trains and called this the "Landover to South End" section in its Maryland Division.