The specimen described was obtained by Philip Darlington in northern Queensland, another member of a work group for the American Museum of Comparative Zoology, the Harvard Australian Expedition (1931–1932) led by William M.

A key to a diagnosis for the species is the proportion of the phalanx bones in the third finger, the ratio of the third to second phalanges is greater than 0.84.

The larger size of the Vespadelus darlingtoni distinguishes it from others of the genus, the forearm length being greater than 33 mm (1.3 in), in particular the similar species V. pumilus (eastern forest bat).

[1] They occur at all elevations in the southern regions, but the range to the north is restricted to the cooler environs at higher altitudes.

[1] Vespadelus darlingtoni is common around the eastern headwaters of the Murray and Murrumbidgee rivers, favouring woodland and other habitat in the agricultural regions.

Vespadelus darlingtoni is observed in small reserves of woodland of rural areas and present at the urbanised landscape.