An oil painting on canvas executed in 1592–1594, it is housed in the Basilica di San Giorgio Maggiore in Venice, Italy.

His earlier paintings for the Chiesa di San Marcuola (1547) and for the Chiesa di San Felice (1559) depict the scene from a frontal perspective, with the figures seated at a table placed parallel to the picture plane.

Tintoretto's painting of 1592–1594, a work of his final years, departs drastically from this compositional formula.

Tintoretto's Last Supper makes use of Mannerist devices in its complex and radically asymmetrical composition.

"The ability of this dramatic scene to engage viewers was well in keeping with Counter-Reformation ideals and the Catholic Church's belief in the didactic nature of religious art.