This is paramount to stratigraphic dating, which requires a set of assumptions, including that the law of superposition holds true and that an object cannot be older than the materials of which it is composed.
To illustrate the practical applications of superposition in scientific inquiry, sedimentary rock that has not been deformed by more than 90° will exhibit the oldest layers on the bottom, thus enabling paleontologists and paleobotanists to identify the relative ages of any fossils found within the strata, with the remains of the most archaic lifeforms confined to the lowest.
The law of superposition was first proposed in 1669 by the Danish scientist Nicolas Steno, and is present as one of his major theses in the groundbreaking seminal work Dissertationis prodromus (1669).
Human-made intrusions and activity in the archaeological record need not form chronologically from top to bottom or be deformed from the horizontal as natural strata are by equivalent processes.
Original stratification induced by natural processes can subsequently be disrupted or permutated by a number of factors, including animal interference and vegetation, as well as limestone crystallization.