Santos was an NJCAA national champion with Iowa Central before joining Santa Fe in the new Colombian Women's Football League in 2017.

One afternoon, César Correa, a friend of her father's, saw her play and recommended that she travel to Bogotá so she could try out for Club Besser, who was looking for female players.

[3][4] After a call from the Colombia women's national team and gathering the support of her family and friends to finance the trip, she moved to Bogotá with Correa.

[6] The team finished the season as NJCAA national champions after she assisted on the lone goal of the championship match against Eastern Florida State College.

Santos began playing for Santa Fe in the National Women's Club Championship in the Amateur Division of Colombian Soccer, a precursor to the full professional league.

[15] In 2018, Santa Fe began their season with a defeat, but roared back with a massive 10–0 victory in their second game, with Santos scoring 4 goals.

[17] After the championship, a conflict arose between the Colombian Soccer Federation and the women's professional and national team players after complaints of sexual and labor abuse, along with homophobic and misogynistic behavior.

[22] She debuted on 7 September 2019 in a 1–0 win against Sporting de Huelva at Estadio Nueva Condomina, coming on for Toni Duggan in the second half.

After starting the season as a substitute, injuries to players likes Virginia Torrecilla and Toni Duggan led to her becoming a regular starter.

[26] On 15 December 2020, she became the first Colombian woman to score in the UEFA Women's Champions League; her goal came during the second leg of their round of 32 match against Servette, a game that ended in a 5–0 victory for Atlético Madrid.

In the Copa de la Reina, they won the final against Real Madrid in a penalty shootout after coming back from being down 2–0 in stoppage time; Santos did not play due to injury.

[32] On 2 April 2024, Washington Spirit announced that they had signed Santos to a three-year contract and that she would join the team after the conclusion of the 2023–24 Liga F season.

[33] In the quarterfinal of the 2023 FIFA Women's World Cup, Santos scored a lob goal against England goalkeeper Mary Earps, in a manner that commentators compared to the lob goal scored by Ronaldinho against England goalkeeper David Seaman in the quarterfinal of the 2002 FIFA Men's World Cup.

[34] With fellow professional footballer Lina Arciniegas, Santos is the co-founder of Cacahuates, a company that creates and distributes nut creams.