Tornado Lou, the local chanteuse, regales the saloon-goers with a sultry ballad ("Když v báru houstne dým"), while the saloon owner Doug Badman tries in vain to woo her.

Two evangelists, Ezra Goodman and his daughter Winnifred, enter the bar attempting to drum up interest in their temperance movement, but the saloon's hard-drinking cowboys scorn them.

Joe, unaware of the developments, is riding on the prairie ("Sou fár tů jů áj méj") until, thanks to a mirage, he discovers that Hogofogo has his own designs on Winnifred.

Their plan begins with Hogofogo, now disguised as a blind piano tuner ("Horácův pohřební blues"), kidnapping Winnifred as a bait to lure Joe to Dead Man's Valley ("Balada Mexico Kida").

Just as he is about to shoot Winnifred, Lemonade Joe enters alive and well; surveying the three dead bodies, he notices their birthmarks and discovers that they are his long-lost siblings.

The entire Kolalok family, including the newly married Winnifred and Joe, ride off into the sunset in a stagecoach as the population of Stetson City cheer.

[8] The academician Anikó Imre noted that "by ridiculing the racist and sexist framework of the Western genre," Lemonade Joe also implicitly satirizes the rigid ideology of the Soviet government in the 1960s.

[9] The cultural anthropologist Cynthia Miller concludes that the film "both glorifies and mocks the wonders of capitalist enterprise, and in so doing, creates a meeting ground between Maysian [i.e. Karl May-like] celebration and contemporary Soviet denunciation of all things West.

In the early 1960s, during Nikita Khrushchev's De-Stalinization of the Soviet Union, Westerns began to reappear in Czechoslovakia, with films such as High Noon, The Big Country, and The Magnificent Seven screened in theatres.

[12] Jiří Brdečka, a prolific Czech screenwriter and satirist,[13] created the Lemonade Joe character in a 1940 serial,[14] a parody of dime novels commissioned by the popular magazine Ahoj na neděli.

[16] Brdečka also wrote a nonfiction work about the American frontier, Kolty bez pozlátka (1956), de-mythologizing the iconic Western figures of Wild Bill Hickok, Billy the Kid, and Jesse James.

[18] The stories also inspired the 1949 stop-motion animation short film Song of the Prairie (where the Lemonade Joe theme song, "Sou Fár Tu Jú Aj Mej", appeared for the first time)[19] as well as two other animated films: Dušan Vukotić's Cowboy Jimmie (Yugoslavia, 1957) and Witold Giersz's Maly Western ("The Little Western," Poland, 1961).

Joe's songs were dubbed by the "Golden Voice of Prague," Karel Gott, and the popular singer Waldemar Matuška was cast for a small role including a solo.

[26] In the mid-1980s, when Mikhail Gorbachev introduced measures to limit Soviet alcohol consumption, critics nicknamed him "Lemonade Joe" in a nod to the film.