Lesotho sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016.

The delegation to Rio de Janeiro consisted of two athletes: sprinter Sello Mothebe and discus thrower Litsitso Khotele.

[4] They were accompanied by chef de mission Lefa Moneri, team manager Makatleho Liau, deputy sports minister Marefuoe Muso, disability sports desk officer Samuel Patsa, National Paralympic Committee of Lesotho secretary-general Jobo Raswoko and coaches Sellolane Tsoaeli and Thabiso Ratsoane.

[12][10][n 2] In January 2017, it was announced that he tested positive by the World Anti-Doping Agency for the banned substance 19-Norandrosterone prior to competing at the Games.

[13][14] A subsequent appeal with the Court of Arbitration for Sport was denied nine months later and Mothebe was banned from competition for four years and his results from Rio de Janeiro were annulled.

[14] Litsitso Khotlele was 32 years old at the time of the Rio Summer Paralympics and these Games were her first significant international competition of her career.