The festival has been held annually since 2008, and screens independent short, documentary and feature films from Israel and abroad.

[1] Lethal Lesbian was co-founded by LGBT activists Anat Nir and Dana Ziv,[2] who organize and produce the festival with feminist Lior Elefant, its artistic director.

It screens short and full-length films of all genres, and in some years, produces a concurrent exhibition of women's art.

[6] In 2018, as Lethal Lesbian celebrated a decade of queer women's film, the mainstream press lauded its staying power "in a landscape in which cultural events have trouble maintaining stability over time".

[7] The festival is one of only few events aimed specifically at women in the LGBT community, and has provided a supportive venue for the works of lesbian filmmakers.