Although Czechoslovakia was a land-locked nation, a floatplane target tug was necessary for a Czechoslovak anti-aircraft artillery training depot in the Bay of Kotor (now in Montenegro) and four were built as the Š-328v (v stood for vodní or water).

13 planes from first production batch were tested as night fighters, armed with four 7,92 mm vz.30 machine guns in the wings and two movable vz.30s for the observer.

At the time of the Munich agreement, which ended the Sudeten crisis the Czechoslovak Air Force had 227 planes in operational units and 87 in training schools and mobilisation depots.

On 23 March 1939, Hungary invaded Slovakia, and in the brief Slovak–Hungarian War, Slovak Š-328s were used for reconnaissance and attack duties against the advancing Hungarian forces.

Data from Tschechoslowakische Flugzeuge : von 1918 bis heute,[15] and Jane's All the World's Aircraft 1934 [16]General characteristics Performance Armament