It was located in the former Fayette County Courthouse until 2012 when the city closed the building for renovation.

[1] Prior to the closing of the building, the Isaac Scott Hathaway Museum moved to a new location on Georgetown Street.

The building has since been renovated into a multi-use commercial, tourist, office and event center.

The building was opened as a museum center in October 2003 under a Memorandum of Understanding between the Lexington-Fayette Urban-County Government (Lexington, Ky.) and the Commonwealth of Kentucky that stipulated the municipal government would spend a "minimum $1,000,000" to renovate the Old Fayette County Courthouse as the Lexington History Museum—an amount that was never fulfilled.

The Lexington History Center was formerly host to several museums: