Although it is related to the Christian Church (Disciples of Christ), it is intentionally ecumenical with almost 50 percent of its enrollment coming from other denominations.

The State of Kentucky began to pressure the school, controlled by a religious body receiving federal funding as a land grant institution under the Morrill Act, to separate portions.

The prior went on to become the state's primary public institution, the University of Kentucky; and the College of the Bible struggled during the presidency of John William McGarvey.

In 1912, after the McGarvey's death, the College of the Bible resumed its affiliation with Transylvania under the leadership of Dr. Richard Henry Crossfield Jr.

In 2010, Lexington Theological Seminary launched a new Master of Divinity program with up to two-thirds of the required classes online.

LTS has in recent years moved most of its instruction online and doesn't require as much physical space as previously.

[6] The seminary also provides continuing education and life-long learning opportunities through The Lay School of Theology (LST @ LTS) and Creating Vital Congregations Webinars.