Lilla Karlsö is a small Swedish island in the Baltic Sea, situated about 3 km (1.9 mi) off the west coast of Gotland and 4.5 km (2.8 mi) from Stora Karlsö; it is part of Eksta socken.
During summer there are tour boats from Djupvik 15 km (9.3 mi) south of Klintehamn.
The island has an area of about 1.6 km2 (0.62 sq mi) and is 66 m (217 ft) high.
There is some old broadleaf forest, which is unique for Gotland, and several rare plants for Sweden such as Lactuca quercina (called 'Karlsösallat' in Swedish), hart's-tongue fern and Petrorhagia prolifera.
Along with neighbouring Stora Karlsö, the island has been designated an Important Bird Area (IBA) by BirdLife International.