Currently a talk show host for Italian private television channel La7, Gruber also served as Member of the European Parliament from 2004 to September 2008 with the Olive Tree centre-left coalition.

In April 2004, Gruber resigned from her position at RAI in protest against the influence on state controlled media by Prime Minister Silvio Berlusconi.

She attacked his "unresolved conflict of interest", and considered that RAI had abandoned its tradition of pluralism in order to support the government's views.

After her resignation from European Parliament, in 2008 Gruber accepted an offer from private TV channel La7, where she took charge of the long-running political talk show Otto e mezzo (it).

She was included in the guest list of Bilderberg Group 2012 edition, at Chantilly, Virginia, as Journalist/Anchorwoman, and was the only journalist allowed at the 2013 meeting in Hertfordshire, England.