She is best known for her role as Sue Charlton in the Crocodile Dundee film series (1986–2001), with the first installment earning her a Golden Globe Award nomination.

In 1987, Kozlowski was nominated for a Golden Globe award in the Best Supporting Actress category for her performance as Sue Charlton.

Also, in 1988, she starred with Bill Paxton, Tim Curry and Annie Potts in Pass the Ammo and the TV miniseries Favorite Son.

Since that time, she has appeared in Almost an Angel (also starring Hogan) in 1990, Backstreet Justice (with Paul Sorvino) and The Neighbor (with Rod Steiger) in 1994, and Village of the Damned in 1995.

She has largely left the acting business because of dissatisfaction with her roles, stating: "These straight-to-video, schlocky films I was getting were giving me an ulcer, basically because I was the only one on the set that cared about anything...