The following is a list of cast members who portrayed or voiced characters appearing in the Spider-Man films produced primarily by Columbia Pictures and later co-produced by Marvel Studios and Sony Pictures Animation.
Tom Holland portrays another iteration of the character set in the Marvel Cinematic Universe (MCU), headlining a trilogy co-produced by Columbia Pictures and Marvel Studios consisting of Spider-Man: Homecoming (2017), Spider-Man: Far From Home (2019), and Spider-Man: No Way Home (2021), the latter of which he is joined by Maguire and Garfield in supporting roles.
Holland also appeared as the character in the MCU films Captain America: Civil War (2016), Avengers: Infinity War (2018), and Avengers: Endgame (2019), and had an uncredited cameo appearance in the Sony's Spider-Man Universe (SSU) film Venom: Let There Be Carnage (2021).
Another iteration of the character is introduced in the SSU film Madame Web (2024).
Several other cast members who recur in the film series and/or within the franchises include Willem Dafoe, Kirsten Dunst, James Franco, Rosemary Harris, Cliff Robertson, Alfred Molina, Thomas Haden Church, Emma Stone, Rhys Ifans, Sally Field, Jamie Foxx, Jon Favreau, Zendaya, Jacob Batalon, Tony Revolori, Marisa Tomei, Brian Tyree Henry, Lauren Vélez, Mahershala Ali, Oscar Isaac and Kimiko Glenn This section includes characters who have appeared in noteworthy roles.