List of number-one singles of 2000 (Australia)

Its data, published by the Australian Recording Industry Association, is based collectively on each single's weekly physical and digital sales.

Fourteen acts gained their first Number 1, either as a lead or featured artist: Macy Gray, Killing Heidi, Chris Franklin, *NSYNC, Bardot, Destiny's Child, Madison Avenue, Bomfunk MC's, Anastacia, Pink, Spiller, Sophie Ellis-Bextor, Wheatus and Baha Men.

Both Madonna and Kylie Minogue earned two Number 1 singles during the year.

Lisa "Left Eye" Lopes and "Jumpin', Jumpin'" by Destiny's Child Songs that peaked at number three include "She Bangs" by Ricky Martin, Lucky by Britney Spears, "Gotta Tell You" by Samantha Mumba, "We Will Rock You" by Five + Queen and "Steal My Sunshine" by Len.

Other hit songs included "Shine" by Vanessa Amorosi (4), Rock DJ By Robbie Williams (4), "It's My Life" by Bon Jovi (5), "Don't Tell Me" by Madonna (8), Let's Get Loud by Jennifer Lopez (9)