Little St Mary's or St Mary the Less is a Church of England parish church in Cambridge, England, on Trumpington Street between Pembroke College's Mill Lane Project development site and Peterhouse.

[2] In the early 1280s Hugh de Balsham, Bishop of Ely, lodged some scholars in the Hospital but to his dismay found soon that the sick and the students could not live in harmony together.

In 1632 Peterhouse built a separate Chapel and St Mary the Less reverted to being solely a Parish Church.

In 1643, after his departure, many of the Church's ornaments and statues were damaged or destroyed by the Puritan iconoclast William Dowsing.

At Little Mary’s, December 29, 30, we brake downe 60 superstitious pictures, some popes, and crucifixes, and God the Father sitting in a chayer, and holding a globe in His hand.In 1741 the church was refitted with wooden panelling, box pews, choir gallery, and the present pulpit.