Some of its most notable regular performers include Audra McDonald (the program's official host), Plácido Domingo, Luciano Pavarotti, Leonard Bernstein, Itzhak Perlman, George Balanchine, Isaac Stern, Nathan Lane, James Galway, Billy Porter, Zubin Mehta, James Naughton, Kurt Masur, Kristin Chenoweth, Jason Isbell, Beverly Sills, Yo-Yo Ma, Renée Fleming, Emma Thompson, Joan Sutherland, Josh Groban, Mikhail Baryshnikov, Patina Miller, the New York City Ballet, the Mark Morris Dance Group, the American Ballet Theatre, the Alvin Ailey American Dance Theater, the New York Philharmonic, and the New York City Opera.

Martin Bookspan served as the program announcer from the premiere of Live from Lincoln Center until his retirement in 2006, at which point Fred Child took over.

On-camera hosts throughout the broadcast's history have included Dick Cavett, Hugh Downs, Sam Waterston, Garrick Utley, Patrick Watson, and Beverly Sills.

Most recently, the host position has been filled by Alan Alda, Alec Baldwin, Lesley Stahl, and currently, Audra McDonald.

[9][10][11] The current team of directors for the program is made up of Habib Azar, Dana Calderwood, Alex Coletti, Matthew Diamond, Brad Fuss, Annette Jolles, Lonny Price, Alan Skog, Glenn Weiss, and Andrew Carl Wilk.