It was formed in 1798 from parts of the Home and Western Districts, and lasted until its abolition in 1850.

The District was formed by an Act of the Parliament of Upper Canada in 1798, and was described as consisting of ...the Counties of Norfolk, Oxford and Middlesex with so much of this Province as lies to the Westward of the Home District, and the District of Niagara, to the Southward of Lake Huron, and between them and a line drawn due north of a fixed boundary (where the easternmost limit of the township of Oxford intersects the River Thames till it arrives at Lake Huron...[1] The townships and counties were thus organized: The district town was Charlotteville (later named Turkey Point), but moved to Tisdale's Mills (later named Vittoria) in 1815.

[3][4] In 1826, the district town was moved to London, and the townships of Rainham and Walpole were moved to Haldimand County in Niagara District because of their distance from London.

[5] The Huron Tract, being developed at the time by the Canada Company, was divided between the London and Western Districts in 1835, with the greater part of the territory in the London District forming the new Huron County.

[17] thus detaching the remainder of its northern part extending to Lake Huron.