A midfielder, Pusineri began his career with Almagro in 1997, and enjoyed successful spells at San Lorenzo and Independiente.
During his three years with Club Atletico San Lorenzo de Almagro, Pusineri made 61 appearances and scored 11 goals.
After failing to make any impact on European soil, Pusineri decided to move back to Independiente in season 2004/2005.
In 2006, Pusineri was signed by Daniel Passarella, who took over from Reinaldo Merlo as the manager of River Plate but returned once again to Independiente in 2007.
During the 2013-14 Championship, Pusineri worked with Adrián Domenech as technical assistant of Claudio Borghi for Argentinos Juniors.