Werner started her career at the Holland Media Group, anchoring news programs about show business.

[1][2] Werner suffered from a lack of oxygen during her birth, leaving her with cerebral palsy resulting in a limp.

[6][10] Werner made her debut as a television host in September 1997, when the news show RTL Actueel premiered.

[1][13][14] Besides, she encouraged viewers of Henny's House Party, a talent show for disabled people, to donate money to a charity.

Her first show there was Alle dieren tellen mee, which was alternately presented by Werner and Anniko van Santen starting in September, about animals.

The former game show was on television in the early evening on weekdays and was hosted before by Paula Udondek, while the latter program had questions about animals and was broadcast on Saturdays.

[24] When rumors reached the media in late 2006 that the network coordinator considered ending Lingo, Prime Minister Jan Peter Balkenende reacted by asking the rhetorical question "How fond are we of presenter Lucille Werner?

"[25] In 2007, Werner also hosted the five-episode spin-off Lingo Bingo Show, in which celebrities competed and raised money for the VUmc Cancer Center Amsterdam.

[39] She became one of the hosts of De Ochtend Show to go, a new online news program by a number of DPG Media newspapers, in December 2018.

She was the host of a 2003–04 concert tour by musicians with a disability and presented the 2005 singing competition Scholierentour for young people.

[48][49] In addition, she created the children's drama series Caps Club, that has three seasons and was broadcast by TROS/AVROTROS and in Belgium by Ketnet in the years 2013–16.

The show about a visually impaired boy nicknamed Cappie and his friends had a cast that included disabled child actors.

[63][64] She was installed on 31 March and became her party's spokesperson for disability policy, long-term health care, caregiving, the Social Support Act 2015, volunteering, media, culture, emancipation, and civilian service.

[66] In the House, she proposed a fixed television channel number for regional broadcasters in order to increase their visibility.

[67] Together with two other parties, she also advocated for appropriating additional funds to the public broadcasting organization to increase the amount of programs with audio description.

[69] When the collapse of the fourth Rutte cabinet triggered a snap election in November 2023, Werner announced she would not run for re-election.

[70] In the second half of the 1990s, Werner bought a historic farm in the North Brabant village Strijbeek, situated close to the border with Belgium, where she lived with her boyfriend.

[71] She married Servaas Snoeijers (born 1969), who works in the media industry, in their place of residence Nederhorst den Berg on 5 June 2009 after being in a relationship with him for five years.