Ludowika Margaretha of Zweibrücken-Bitsch (19 July 1540, Ingwiller – 15 December 1569, Bouxwiller), was the only child and heiress of Count James of Zweibrücken-Bitsch (born: 19 July 1510; died: 22 March 1570) by his wife Catherine, born Countess of Honstein zu Klettenberg.
Her father, Count James of Zweibrücken-Bitsch (1510–1570), was the last male member of the House of Zweibrücken.
Bitsch was formally a fief of the Duchy of Lorraine and could in theory only be inherited in the male line.
However, he immediately introduced the Lutheran faith in his newly gained territory and this made the powerful and Catholic Duke of Lorraine unhappy.
In a treaty signed in 1606, it was agreed that Bitsch would revert to Lorraine and Hanau-Lichtenberg would retain Lemberg.