[1] Lane was born in Hackney, London, son of Harry Charles Lupino (1867–1925), part of the Hook family who adopted the surname 'Lupino.'

Hammons sent his chief producer Jack White to see Lane on stage: "He was working in New York in The Mikado, Gilbert and Sullivan.

During his first year at Educational, Lane found time to produce and star in The Hollywood Music Box Revue, which enjoyed an unusually long run (by local standards) of 19 weeks.

Some of the girls featured in the show went on to screen careers, including Lupe Vélez, Nancy Carroll, and Marion Byron.

"[7] Lane demonstrated that he knew his business, and his comedies successfully displayed his agility and versatility: in one film he played 27 characters (Only Me, 1929).

In December 1929 the New York Daily News reported that Lupino Lane "is deserting Hollywood for a six-month engagement at the Hippodrome in London.

"[10] A subsequent Film Daily report added that Lane was leaving Hollywood for his native England, to form his own production company, which was confirmed in a European dispatch from January 1930: "Lupino Lane, who is now appearing at the Glasgow Alhambra, intends setting up an organisation of his own outside London, to make British comedies, and his immediate plans include four features and twelve two-reel comedies a year.

"[11] Lane's surprise swan song in America was a Vitaphone short called Evolution of the Dance, released in February 1930 as a two-reel Technicolor special, even though the running time (12 minutes) barely exceeded one reel.

With Sir Oswald Stoll, Lane co-produced Twenty to One, written by L. Arthur Rose and Frank Eyton with music by Billy Mayerl, on the West End.

[1] Me and My Girl, the follow-up show, written by Rose and Douglas Furber with music by Noel Gay, was an even bigger hit.

It featured a hit song and dance routine from Lane called "The Lambeth Walk", which became popular throughout Europe in the late 1930s.

[14] The Me and My Girl film was released by Metro-Goldwyn-Mayer in January 1940 under the title The Lambeth Walk, purely on the popularity of the dance craze.

[citation needed] In 1946, after it sustained damage during World War II, he purchased the shell of the Gaiety Theatre in London to rescue it from dereliction, intending to produce comedies.

[18] A commemorative blue plaque was erected to Lupino Lane on 15 June 2014 at his former home 32 Maida Vale, Paddington, by The Music Hall Guild of Great Britain and America.