The 1,398-hectare (3,450-acre) park is situated approximately 30 kilometres (19 mi) southeast of the Melbourne central business district, adjacent to the suburb of Lysterfield in the City of Casey.

When combined with the adjacent Churchill National Park, the two parks comprise 1,668 hectares (4,120 acres) in the Dandenong Valley and the Dandenong Ranges that are a haven for native birds, mammals and reptiles, and provide recreational opportunities.

[1] Lysterfield Park was the venue for mountain biking events of the 2006 Commonwealth Games.

The trails are well signposted and give clear indication of the level of difficulty they present to the rider.

Trail management over recent years 2012 - 2015 has led to vastly improved drainage, usage of natural and man-made features and as a result trail conditions are much improved.