Influenced by the fashionable beliefs of Physiognomy of the 19th century, Duchenne wanted to determine how the muscles in the human face produce facial expressions which he believed to be directly linked to the soul of man.
He published his findings during 1862, together with extraordinary photographs of the induced expressions, in the book The Mechanism of Human Physiognomy (Mécanisme de la physionomie humaine).
It is these notions that he sought conclusively and scientifically to chart by his experiments and photography and it led to the publishing of The Mechanism of Human Physiognomy during 1862[4] (also titled, The Electro-Physiological Analysis of the Expression of the Passions, Applicable to the Practice of the Plastic Arts.
[6] He worked with a talented, young photographer, Adrian Tournachon, (the brother of Felix Nadar), and also taught himself the art in order to document his experiments.
Duchenne's experiments for the aesthetic section of the Mechanism included the use of performance and narratives which may well have been influenced by gestures and poses found in the pantomime of the period.
He believed that only by electroshock and in the setting of elaborately constructed theatre pieces featuring gestures and accessory symbols could he faithfully depict the complex combinatory expressions resulting from conflicting emotions and ambivalent sentiments.
These melodramatic tableaux include a nun in "extremely sorrowful prayer" experiencing "saintly transports of virginal purity"; a mother feeling both pain and joy while leaning over a child's crib; a bare-shouldered coquette looking at once offended, haughty and mocking; and three scenes from Lady Macbeth expressing the "aggressive and wicked passions of hatred, of jealousy, of cruel instincts", modulated to varying degrees of contrary feelings of filial piety.
To help him locate and identify the facial muscles, Duchenne drew heavily upon the work of Charles Bell, although he did not share the Scottish anatomist's interest in the expressions found in insanity.
The exact imitation of nature was for Duchenne the sine qua non of the finest art of whatever age, and although he praised the ancient Greek sculptors for unquestionably attaining an ideal of beauty, he nevertheless criticized them for their anatomical errors and failure to attend to the emotions.