Mézières-en-Brenne (French pronunciation: [mezjɛːʁ ɑ̃ bʁɛn] ⓘ) is a commune in the Indre department in central France.

It is located is at the heart of the Brenne regional natural park.

The Brenne is one of France's most important wetlands.

A large diversity of natural habitats is responsible for an abundance of wildlife of which the best known are the birds: purple heron, black-necked grebe, bittern, marsh harrier, whiskered tern, short-toed eagle.

The Brenne is also Europe's most important site for the European pond tortoise.