During its service it provided crucial logistical support to the Allied war effort on the Macedonian front and the Southern Russia intervention.

On 5 November 1914, the Ottomans entered the conflict on the side of the Central Powers, prompting Britain to void the Cyprus Convention and annex the island, as the two states were now at war.

By the time of the British occupation of the island, Cypriot mules had already cemented their reputation as both sturdy pack animals and an alternative to polo ponies.

[2] On 24 April 1916, the commander of the British Salonika Army, Bryan Mahon, stated that for his advance towards the Greco–Serbian frontier to succeed, the recruitment of 1,676 pack animals and 1,232 muleteers per division was needed.

[3] The rough terrain, limited infrastructure and underdeveloped railway network of Macedonia necessitated the use of pack animals for military logistics.

As of 6 November, 3,496 Cypriots had joined the corps in the capacity of muleteers, saddlers, farriers, coachmen, foremen and interpreters; signing up for 12 month contracts.

[7][8] The ranks of the Macedonian Mule Corps personnel were distinguished by an arm brassard bearing the letters "MMC" and a cap badge.

[9] A crucial factor in the drive's success was the high wages (90 drachmas per month[10]), free food and clothing offered, as well as the fact that the muleteers were registered as camp followers under the Army Act.

[12] During the course of the campaign, Cypriot muleteers were tasked with transporting food, weapons, ammunition and water to the front as well as carrying injured soldiers back and working on road construction.

The Corps played a crucial role in the logistics of the British and French armies on the Macedonian Front, contributing to the eventual Allied victory.