[2] Prespakis has three siblings: twin sister Annalea, Georgie and Jimmy;[1] She attended Gisborne Secondary College and supported Essendon growing up.

[3] Prespakis began playing football at four years old through the Auskick program in Romsey, north-west of Melbourne.

[4] In 2017, Prespakis played in the premiership-winning Calder Cannons side in the TAC Cup Girls.

[5] She competed at the 2017 AFL Women's Under 18 Championships for Vic Metro and was named in the All-Australian squad.

[7] In October, Prespakis was named in the 2018 AFLW Academy squad as one of twenty-nine seventeen-year-old prospects.

[9] She was again named in the TAC Cup Girls Team of the Year and tied with Nina Morrison for the league best and fairest.

She again competed at the 2018 AFL Women's Under 18 Championships; she shared the competition's most valuable player award with Morrison on 16 votes apiece, won Vic Metro's most valuable player award and was named in the All-Australian side.

[14] The following round, she was nominated for the 2019 AFL Women's Rising Star award after kicking one goal and amassing 20 disposals, seven clearances and nine contested possessions against Adelaide.

[42] She later said that she had turned to Carlton AFL co-captain Patrick Cripps at times during the season for advice on how to deal with taggers.

[51][52] She was best afield upon her return against Geelong in round 6 with 27 disposals and a goal, despite injuring her left elbow in the first quarter,[53] and again won selection in womens.afl's Team of the Week.