The Main-Neckar Railway (German: Main-Neckar–Eisenbahn, MNE) is a main line railway west of the Odenwald in the Upper Rhine Plain of Germany that connects Frankfurt am Main to Heidelberg via Darmstadt, Bensheim and Weinheim.

The railway line is part of the networks served by the Rhein-Main-Verkehrsverbund and Verkehrsverbund Rhein-Neckar.

Baden requested that the line be built to 1600 mm broad gauge but could not gain agreement for this.

The line was opened for the scheduled traffic in sections: Until the Main-Neckar Bridge in Frankfurt (at the site of today's Friedensbrücke) was completed on opened to traffic on 15 November 1848, rail services ran into the Mainspitze depot and reversed to the old Sachsenhausen station.

It was in the (present) Willy-Brandt-Platz on the corner of Münchner Straße and Gallusanlage, south of the Taunus station.

[13] The Main-Neckar Railway brought jobs and income for the people of the Bergstrasse and the western Odenwald.

Only men with impeccable reputation, able to satisfy a thorough examination that they were in perfect health, would be allowed to make the substantial deposit for the coveted dress uniform of railway workers.

In the following years, the line was constantly developed, modernised and adapted to increasing traffic and the changing needs of passengers.

Today, the Main-Neckar line shares the load of north-south traffic with the Mannheim–Frankfurt railway, which runs further to the west in the Rhine valley, from Frankfurt, bypassing Darmstadt, via Groß-Gerau to Mannheim and Worms.

On the section between Darmstadt and Mannheim/Heidelberg, the Main-Neckar Railway has reached the limit of its capacity: 250 trains run on the line in each direction every day.

Regional-Express trains stop only in Frankfurt, Langen, Darmstadt, Bickenbach, Bensheim, Heppenheim, Hemsbach, Weinheim and Ladenburg.