Majdalena is a municipality and village in Jindřichův Hradec District in the South Bohemian Region of the Czech Republic.

It has about 500 inhabitants.

Majdalena lies approximately 24 kilometres (15 mi) south-west of Jindřichův Hradec, 28 km (17 mi) east of České Budějovice, and 129 km (80 mi) south of Prague.

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