[1][2] Grand Rapids Wanderers and Muskegon Risers II were the first two member clubs announced[3] with the Omaha Kings FC and Sunflower State FC named the same week.

[4][5] Wichita Wings II and Springfield Demize were also added to the inaugural MASL3 lineup.

[6][7][8] With the ongoing COVID-19 pandemic just four teams (Grand Rapids and Muskegon sat out) competed in the inaugural season of 2020–21.

Omaha Kings FC finished the six-game regular season unbeaten and went on to capture the Heartland Invitational Cup with victories over Wichita Wings II (11-8) and Sunflower State FC (13-7).

[9] In September 2024, Major Arena Soccer League announced the unification of several former PASL member clubs to join MASL 3 in addition to a new women's league (MASL W).