[4] Mameluke finished second to an unnamed filly by Partisan (later named Donna Maria), but the judge mistakenly awarded second place to his stable companion Glenartney, who was carrying the same colours.

He was next scheduled to take part in a match race on 30 April, but collected the £200 forfeit money without running when his opponent, a filly later named as "Mulebird"[5] failed to appear.

Glenartney was also in the field and started at 5/1 but Lord Jersey, who owned both colts, declined to announce a public preference for either and insisted that each horse would run on its merits.

With half a mile to run, most of the runners were struggling but Mameluke had moved easily into second place and the two Jersey colts raced into the straight clear of the rest of the field.

After running alongside Glenartney as if taking part in an exercise gallop at home, Robinson sent Mameluke into the lead and he won comfortably, while Edwards seemed to make little effort to challenge him.

[2] It was widely believed that Glenartney had been "pulled"[6] (deliberately restrained) by his jockey in the closing stages to allow his stable companion to win: it was known that Edwards had placed a large bet on Mameluke.

[9] When the starter, Mr. Lockwood finally allowed the race to begin, a filly named Matilda was well in front of the other runners, while Mameluke, who had become increasingly upset[2] by the situation and was facing in the wrong direction, was left at least sixty yards behind.

[10] A less cynical view of the 1827 St Leger was offered by Henry Hall Dixon ("The Druid") who suggested that the trouble at the start was caused by the large number of inexperienced and incompetent jockeys, and that Mameluke's defeat was primarily due to the poor conditioning he had received after his transfer to Sykes.

[1] A writer in The Sportsman on the other hand, laid the blame for Mameluke's defeat on Chifney for a lack of alertness at the start and poor judgement in making up the lost ground too quickly.

For the second successive year at Newmarket's Second Spring meeting his owner was able to claim forfeit money for a match race, when his scheduled opponent, the 1826 Epsom Oaks winner Babel (formerly known as Lilias[13]) was withdrawn.

In June he came second to the favourite Zinganee in a very strong field[16] for the Ascot Gold Cup, finishing ahead of the Classic winners Cadland (Derby), The Colonel (St Leger) and Green Mantle (Oaks).