Adult males can also change their skin color from pale brown to jet black when they engage in mate-calling to attract females.

It also has a well-defined pale inguinal stripe, bandlike concentrations of melanophores along the anterior arms, and dark pigmentation on the metatarsal and subarticular tubercles of its toes.

[6] The frog can live anywhere ranging from sea level to extremely high elevations and prefers to reside around rocky streams in valleys, mountain slopes, or undisturbed moist montane forests.

[7] The streams are typically narrow and shallow with slow, clear running waters and contain deeper pools where frogs can deposit and develop their tadpoles.

Frogs will sometimes transport their tadpoles from these streams to live in other pools such as phytotelmata if a large number of predators exist nearby.

[1] However, a significant number of frogs have suffered regional habitat loss and degradation through pollution, deforestation, and shifting cultivation.

One study from Venezuela, proposed direct conservation measures include forest preservation and strict regulations on agrochemical products used on coffee and cocoa plantations near streams.

[8] Adult Trinidad poison frogs often feed on arthropods and small insects such as Drosophila (D. hydei and D. melanogaster) and Callosobruchus chinensis.

[4] Adult female Trinidad poison frogs lay their eggs near streams following increased humidity from rainfall.

[2][4] The Trinidad poison frog and its tadpoles are commonly preyed on by a killifish species (Anablepsoides hartii) and shrimp of the genus Macrobrachium.