Manshu-in ((曼殊院), Manshu-in), also known as the Manshuin Monzeki, is a Tendai monastery located near the Shugakuin Imperial Villa at Sakyō-ku, Ichijo-ji, Takenouchi-cho, in northeast Kyoto, Japan.

In the early Edo period the monastery moved to its current site.

The main temple (Great Shoin) dates from the early Edo period, and is now listed as an Important Cultural Property.

It contains a Waterfall Room with slides by Kanō Tan'yū 狩野探幽 (1602–1674), and Snowy Scenes Room with screen pictures by the same artist and shelves much like those in the Katsura Imperial Villa.

The major garden is in the Karesansui (枯山水) style, and now designated as an eminent scenery; it contains a notable Pinus pentaphylla tree, now about 400 years old, set within an "island" on a stream of white sand.