Don Manuel Luis Gayoso de Lemos y Amorín KOM OTS (May 30, 1747 – July 18, 1799) was the governor of Spanish Louisiana from 1797 until his death in 1799.

At age 23 Manuel Gayoso de Lemos joined the Spanish Lisbon Regiment as a cadet (1771), and he was commissioned ensign (sub-lieutenant) the following year.

Gayoso de Lemos continued to encourage American settlement on Spanish soil, especially by Catholics, notably the Irish and the Scots, and by those who brought significant property.

While in Natchez, Gayoso de Lemos used the greed of a number of Americans, notably General James Wilkinson and Philip Nolan, to help limit the growth of the United States.

His first act was to issue a Bando de Buen Gobierno (Edit of Good Government) and to send a list of instructions to commandants of all posts concerning land grants.

In addition to increasing formal church membership, it attempted to coerce people to give up unnecessarily working on Sundays and holy days.

Gayoso de Lemos died in New Orleans of yellow fever on July 18, 1799, and his remains were interred in the Saint Louis Cathedral.