Margaret Stuart (24 December 1598 – March 1600) was the second daughter of King James VI of Scotland and Anne of Denmark.

This letter was sent to Robert Bethune, laird of Balfour in Fife:"We greet you well: having appointed the baptism of our dearest daughter to be here at Holyrood House, upon Sunday, the 18th of April next, in such honorable manner as that action craved: we have therefore though good right effectually to request and desire you to send us such offerings and presents against that day, as is best then in season and convenient for that action, as you regard our honor and will merit our special thanks.

So, not doubting to find your greater willingness to pleasure us herein, since you are to be invited to take part of you own good cheer, we commit you to God.

[7] James paid £400 Scots for clothes made by the tailor Peter Sanderson for Margaret and her sister Elizabeth to wear at the baptism.

Alexander Barclay, apothecary, and the German physician Martin Schöner were called to "supply certain drugs, medicaments, and other gear, for the use of the Lady Margaret, during the time of her sickness" and assist the infant, but to no avail.